Fig. 5

RT-PCR of cancerous-related genes BCL-2, PARK, TARC, and LIF of HCT-116 cells treated by RE 245 (Accession No.215810), and RE 280 (Accession No.215848), (relative to 5-FU treated cells) the gene expression was normalized relative to GAPDH and performed in triplicate, For the quantification, the formula RQ = 2-ΔΔct was used. Statistical analysis was performed using T-test. *p < 0.05, **P < 0.01 statistically significant differences from the positive control. Untreated cells were used as control and HCT-116 cells treated by 5-FU were used as positive control