Fig. 4

RT-PCR of IL-2, IL-4, IL-6, CD1A, and CD1B of HCT-116 cells treated by RE 245(Accession No.215810), and RE 280 (Accession No.215848), (relative to 5-FU treated cells) the gene expression was normalized relative to GAPDH and performed in triplicate, For the quantification, the formula RQ = 2-ΔΔct was used. Statistical analysis was performed using T-Test. *p < 0.05, **P < 0.01, ***P < 0.001 statistically significant differences from the positive control. Untreated cells were used as control and HCT-116 cells treated by 5-FU were used as positive control