Fig. 3

Determination effect of Limosilactobacillus fermentum RE 245 (Accession No.215810), Limosilactobacillus fermentum RE 280 (Accession No.215848), and 5-FU on HCT-116 cells. (A) represent flow cytometric analysis results of untreated control and treated HCT-116 cells, Lower left column: annexin VI⁻/PI⁻ (viable cells), lower right column: annexin Vi⁺/PI⁻ (early apoptotic cells), upper right column: annexin Vi⁺/PI⁺ (late apoptotic cells) and upper left column annexin VI⁻/PI⁺ (necrotic cells). (B) Fluorescent microscopy of Hoechst 33,342 staining of untreated HCT-116 cells, treated HCT-116 by 5-FU, RE245, and RE280, respectively. Cell Morphological changes and induction of apoptosis were indicated by arrows with numerous fragmented micronuclei (white), apoptotic body formation (yellow), apoptotic cells with membrane blebbing (red), and secondary necrotic cells (green). (C) Bar plots demonstrate quantitative alterations (viable, necrosis, and early/late apoptosis) of treated/untreated HCT-116 cells from flow cytometry analysis