Table 1 Biochemical profile of the API-50 CH/API-20 NE kit (bioMérieux) of the two representatives H. coagulans isolates

1

Glycerol

+

+

1

ONPG: ß-galactosidase

(Ortho NitroPhenyl-ßDGalactopyranosidase)

-

-

2

Erythritol

-

-

2

ADH: Arginine DiHydrolase

-

-

3

D-Arabinose

-

-

3

LDC: Lysine DeCarboxylase

-

-

4

L-Arabinose

+

+

4

ODC: Ornithine DeCarboxylase

-

-

5

Ribose

+

+

5

CIT: CITrate utilization

-

-

6

D- Xylose

+

-

6

H2S: H2S production

-

-

7

L-Xylose

-

-

7

URE: UREase

-

-

8

Adonithol

-

-

8

TDA: Tryptophane DeAminase

+

+

9

Methyl xyloside

-

-

9

IND: INDole production

-

-

10

Galactose

+

+

10

VP: acetoin production (Voges Proskauer)

+

+

11

D-Glucose

+

+

11

GE: GELatinase

-

-

12

D-Fructose

+

+

12

GLU: fermentation / oxidation (GLUcose)

-

-

13

D-mannose

+

-

13

MAN: fermentation / oxidation (MANnitol)

-

-

14

Sorbose

-

+

14

INO: fermentation / oxidation (INOsitol)

-

-

15

Rhamnose

+

-

15

SOR: fermentation / oxidation (SORbitol)

-

-

16

Dulcitol

-

-

16

RHA: fermentation / oxidation (RHAmnose)

-

-

17

Inositol

-

-

17

SAC: fermentation / oxidation (SACcharose)

-

-

18

Mannitol

-

-

18

MEL: fermentation / oxidation (MELibiose)

-

-

19

Sorbitol

+

+

19

AMY: fermentation / oxidation (AMYgdalin)

-

-

20

Methyl-D-mannoside

+

+

20

ARA.: fermentation / oxidation (ARAbinose)

-

-

21

Methyl-D-glucoside

-

+

21

NO₂: Reduction of nitrates to nitrites

-

-

22

N-acetyl-glucosamine

+

+

23

Amygdalin

+

+

24

Arbutin

+

+

25

Esculine

+

+

26

Salicin

+

+

27

Cellobiose

+

+

28

Maltose

+

+

29

Lactose

+

+

30

Melibiose

+

+

31

Sucrose

+

+

32

Trehalose

+

+

33

Inulin

-

-

34

Melizitose

-

-

35

D-raffinose

-

+

36

Starch

+

+

37

Glycogen

-

-

38

Xylitol

-

-

39

Gentibiose

+

+

40

Turanose

+

+

41

Lyxose

-

-

42

Tagatose

-

-

43

D-fucose

-

-

44

L-fucose

-

-

45

D-Arabitol

-

-

46

L-Arabitol

-

-

47

Gluconate

+

+

48

2, Keto-gluconate

+

-

49

5, keto-gluconate

+

-