Fig. 1

A) Microscopic Examination: The left panel depicts Gram-positive bacilli, and the right panel highlights endospore staining. Red arrows indicate the characteristic of spore production observed at the terminal ends of the bacterial cells. B) Phylogenetic Analysis: The tree, derived from 1,525 bp of the 16S rRNA gene sequence. The tree was constructed using the Maximum Likelihood method in MEGA-11 with 1,000 bootstrapping. C) Biochemical Characteristics: The top panel presents the distinct biochemical profiles of API-50 CHB and the bottom panel presents the biochemical profiles of API-20 NE of the two bacterial isolates