Table 1 Protocols for thermal cycling in genetic research. The thermocyclic patterns for this approach involve the subsequent steps listed below

• The initial denaturation process was conducted at a temperature of 96°C for a period of 5 minutes.

• Following that, 27 cycles were carried out, each consisting of: • Denaturation at 96°C for 2 minutes.

• Annealing at a temperature of 67°C for70 seconds.

•Extension at a temperature of 72°C for 5 minutes.

•Lastly, a last stage of:

The experiment concluded with a final phase carried out at a temperature of 72°C for a continuous period of 10 minutes.