Fig. 6

A) Comparison of negatively-selected conditionally essential genes in P. asiatica JR11 with previously reported conditionally essential genes of P. aeruginosa PGPR2, P. simiae WCS417r, and Pseudomonas sp. WCS365, providing insights into common and unique colonization fitness determinants identified in Arabidopsis thaliana and corn root systems. The Venn diagram was generated using the Multiple List Comparator (www.molbiotools.com/listcompare.html). B) Circular heatmap illustrating the distribution of colonization-depleted genes essential for plant root colonization in identified Pseudomonas spp. The heatmap displays the following functional categories: E - Amino Acid metabolism and transport, G - Carbohydrate metabolism and transport, D - Cell cycle control and mitosis, N - Cell motility, M - Cell wall/membrane/envelop biogenesis, H - Coenzyme metabolism, V - Defence mechanisms, C - Energy production and conversion, S - Function Unknown, P - Inorganic ion transport and metabolism, U - Intracellular trafficking and secretion, I - Lipid metabolism, F - Nucleotide metabolism and transport, O - Post-translational modification, protein turnover, chaperone functions, L - Replication and repair, Q- Secondary Structure, T- Signal transduction, K -Transcription, J - Translation and “–“ indicating no functional COG annotation for the conditionally essential genes