Fig. 1

A schematic representation of the overall experimental workflow for identifying fitness determinants in P. asiatica JR11 during corn root colonization using transposon insertion sequencing. The figure illustrates the novel approach of this study, which involves serially propagating the input population through three enrichment cycles. Following this, DNA extraction and high-throughput sequencing of both input and output populations are performed using the Ion Torrent platform to identify and functionally characterize the conditionally essential genes