Table 1 Ingredients of optimized food-grade media (FGM)

Glucose

-

20

20

20

20

20

20

20

Yeast peptone standard type F

-

25

25

25

25

25

25

25

Proteose peptone No. 3

10

-

-

-

-

-

-

-

Beef extract

10

-

-

-

-

-

-

-

Yeast extract

5

-

-

-

-

-

-

-

Dextrose

20

-

-

-

-

-

-

-

Tween 80

1*

1*

1*

1*

1*

1*

1*

1*

Triammonium citrate

2

-

-

-

-

-

-

-

Trisodium citrate

-

2.1

-

2

-

2

2.1

2

Sodium acetate

5

5

-

5

5

-

-

-

Sodium acetate 3 M

-

-

-

-

-

-

5

-

magnesium sulfate

0.1

0.1

0.2

0.3

0.2

0.3

0.1

0.2

Manganese (II) sulfate

0.05

-

-

-

-

-

-

-

Dipotassium phosphate

2

-

-

2

-

-

-

2