Assessment of biofilm-forming capacity and multidrug resistance in Staphylococcus aureus isolates from animal-source foods: implications for lactic acid bacteria intervention

Table 1 Details of primers used in the study

Genes		Primer sequence (5’-3’)	Annealing temp	Amplicon size (bp)	Reference
*S. aureus* (genus specific) gene	16S rRNA	F: CAG CTC GTG TCG TGA GAT GT	55 °C	420	(Strommenger et al. 2003)
*S. aureus* (genus specific) gene	16S rRNA	R: AAT CAT TTG TCC CAC CTT CG	55 °C	420	(Strommenger et al. 2003)
*S. aureus* (species specific) gene	nuc	F: GCG ATT GAT GGT GAT ACG GTT	55 °C	279	(Brakstad et al. 1992)
*S. aureus* (species specific) gene	nuc	R: AGC CAA GCC TTG ACG AAC TAG C	55 °C	279	(Brakstad et al. 1992)
*S. aureus biofilm forming gene*	icaA	F: GAC CTC GAA GTC AAT AGA GGT	56 °C	814	(Diemond-Hernández et al. 2010)
	icaA	R: CCC AGT ATA ACG TTG GAT ACC	56 °C	814	(Diemond-Hernández et al. 2010)
	icaD	F: AAA CGT AAG AGA GGT GG	45 °C	381	(Vasudevan et al. 2003)
	icaD	R: GGC AAT ATG ATC AAG ATA C	45 °C	381	(Vasudevan et al. 2003)

ISSN: 1869-2044